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产品展示 > 其它产品HyFect DNA转染试剂
产品名称:HyFect DNA转染试剂
产品简介:Low cell toxicity and non-liposome reagent optimized for DNA delivery.
 

Package
        500 μl/vial
Protocol
        1, Cell preparation: Cells should be seeded before 16 to 20 hours prior to transfection with around 70% confluency. The medium should be refreshed 30 mins before transfection. Usually, culture media with serum does not affect transfection.
        2, DNA preparation: DNA plasmid for transfection should be with high purity (A260/A280=1.8~1.9) to ensure efficient transfection mixture preparation.
        3, Mixture preparation: Guideline of DNA plasmid and HyFect DNA transfection reagent amount and ratio refer to the following table 1. In brief, plasmid and transfection reagent are diluted in serum-free culture medium for 5 mins then mixed together and tip gently for another 25 mins.
        4, Transfection: Add mixutures into cell culture dish/plate, the mixture could be removed after 6 to 48 hours and refilled with culture medium.

 
Storage
        Store at -20°C (non-frost-free).
Notice 
        This reagent is for research use only.