Description

       The Bst DNA polymerase is part of the DNA polymerase from Bacillus stearothermophilus expressed in E. coli, which is obtained through isolation and purification. The enzyme has 5’'-3' DNA polymerase activity, but does not have 5'- 3' exonuclease activity. Compared with other DNA polymerase, it has high thermal stability, chain replacement activity and polymerase activity, so it is more suitable for isothermal amplification, such as loop mediated isothermal PCR (LAMP), and 68℃DNA sequencing (this condition is especially suitable for GC rich templates and nanogram level templates). This product cannot be used for thermal cycle sequencing or conventional PCR.

        The enzyme activity is defined as the amount of enzyme required to mix 10 nmoL dNTP into acid insoluble sediment within 30 minutes at 65℃.

Appearance

        Colorless and clear liquid

Package

        2000U/Vial

Stocking buffer

   Stable at -20℃for 36 months.